Zeiss Axiovert 200M

An inverted fluorescence microscope with motorized Z-axis acquisition and a filter wheel with DAPI, GFP, Texas Red and bright field. Objective lenses include Plan-Noefluar 10x/0.3 NA, EC Plan-Neofluar 20x/0.5, LD Achro-Plan 40x/0.6, and Plan-Apochromat 63x /1.4 oil. The system is equipped with sensitive Retiga-SRV (Q-Imaging) CCD camera for high resolution/low-light detection and with Slidebook, the advanced acquisition software (3i, Intelligent Imaging Innovations). Good for fixed cell specimens and chromosome/karyo-type imaging.

 

 

Objectives

  • Plan-Noefluar 10x/0.3 NA
  • EC Plan-Neofluar 20x/0.5
  • LD Achro-Plan 40x/0.6
  • Plan-Apochromat 63x /1.4 oil

 

Applications

Karyotyping

Chromosome painting on mouse embryonic stem cells (Chr 8, green and Chr 11, red).
I. Tereshchenko, J.A. Tischfield

 

Live Cell Imaging

Tra-1-60 positive colonies (green) derived from ectopic expression of hOct4, hSox2, hKlf4 and hc-Myc transcription factors in human foreskin fibroblasts.
N. Sun, J.A. Tischfield