An inverted fluorescence microscope with motorized Z-axis acquisition and a filter wheel with DAPI, GFP, Texas Red and bright field. Objective lenses include Plan-Noefluar 10x/0.3 NA, EC Plan-Neofluar 20x/0.5, LD Achro-Plan 40x/0.6, and Plan-Apochromat 63x /1.4 oil. The system is equipped with sensitive Retiga-SRV (Q-Imaging) CCD camera for high resolution/low-light detection and with Slidebook, the advanced acquisition software (3i, Intelligent Imaging Innovations). Good for fixed cell specimens and chromosome/karyo-type imaging.
- Plan-Noefluar 10x/0.3 NA
- EC Plan-Neofluar 20x/0.5
- LD Achro-Plan 40x/0.6
- Plan-Apochromat 63x /1.4 oil
Chromosome painting on mouse
embryonic stem cells
(Chr 8, green and Chr 11, red).
I. Tereshchenko, J.A. Tischfield
Live Cell Imaging
Tra-1-60 positive colonies (green)
derived from ectopic expression of hOct4,
hSox2, hKlf4 and hc-Myc transcription factors
in human foreskin fibroblasts.
N. Sun, J.A. Tischfield